Description
Principle of the assay: The kit uses a double?antigen sandwich enzyme?linked immunosorbent assay (ELISA) to analyze the existence or not of Human Anti-IF in samples. Add sample to wells pre?coated with one Human Intrinsic Factor antigen, at same time add HRP?conjugated Human Intrinsic Factor antigen to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human Anti-IF in the samples is then determined by comparing the O.D. of the samples to the CUT OFF